Specialized hydrocarbonoclastic bacteria (SHCB), which use limited carbon sources with a preference for petroleum hydrocarbons, were found. Petroleum microbiology is a branch of microbiology that deals with the study of microorganisms that can metabolize or alter crude or refined petroleum products. These microorganisms, also called hydrocarbonoclastic microorganisms, . ” Most Hydrocarbonoclastic Bacteria in the Total Environment are Diazotrophic, which. The marine hydrocarbonoclastic bacterium Alcanivorax borkumensis is able to degrade mixtures of n-alkanes as they occur in marine oil spills. However.

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Growth of microbes on hydrocarbons can be limited by available surface area of the water-oil interface. Physical and chemical approaches are capable of removing a broad spectrum of contaminants but the main disadvantage of these methods lie in the increased energy consumption and the need of additional chemicals Godheja et al. Crude oil consumption by individual bacterial species was measured quantitatively in batch cultures under the conditions specified above.

A previous study estimated that 60 mg Hydrlcarbonoclastic was needed for the microbiological consumption of 1 g hydrocarbons 9. The pure isolated bacteria strains were identified morphologically and on the basis of their biochemical reactions in the Department of Biological Sciences, NDA.

Support Center Support Center. Degradation of aliphatic hydrocarbons. All the five bacteria isolates with potentials for hydrocarbon degradation did not exhibit lag phase of growth Fig 1 which might be attributed to their previous association with the hydrocarbon contaminated environment of the effluent drains from the refinery. Oil-utilizing bacterial species from oil-contaminated sites in Kuwait selected for this study.

Important role of these polymeric byproducts in the conversion of hydrocarbons to bioavailable form is principally responsible for their hydrocarbonoclastic potential in the soil. Neptunomonas naphthovorans degrade polyaromatic hydrocarbons PAH especially naphthalene. Microbiology of oil-contaminated desert soils and coastal areas in the Bacterix Gulf. Bioremediation potentials showed that the mixed culture consortium MCC is more preferable for bioremediation than the pure culture of individual isolates.


One developed system uses Pseudomonas fluorescens HK44 to quantitatively assay for naphthalene using bioluminescence. Survey of petroleum- degrading bacteria in coastal waters of Sunderban Biosphere Reserve. The gel electrophoresis profiles in Fig.

Petroleum microbiology – Wikipedia

Therefore, when nitrogenous compounds and nitrogen gas are simultaneously available, diazotrophs prefer to utilize the former, which explains why nitrogen fertilizers enhance bacterial hydrocarbon bioremediation, as commonly reported, even though most hydrocarbonoclastic bacteria are diazotrophic. Culture-independent approach DGGE showed high diversity in the microbial community in all the studied samples with bateria clear correlation with the hydrocarbon pollution rate.

The results of sequencing of the nifH gene bands in Fig. Hydrocarbohoclastic for oily desert soil.

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Larger oil droplets cannot be taken up by bacterial cells. Not all hydrocarbonoclasic microbes depend on hydrocarbons to survive, but instead may use petroleum products as alternative carbon and energy sources.

Table 3 shows the quantitative data of oil consumption by the individual bacterial species in the presence and absence of NaNO 3 in the medium hydrocarobnoclastic nitrogen gas in the head space. They persist in nature for long period of time and cause hazardous effects on flora and fauna of terrestrial and aquatic ecosystems. Hydrocarbon degradation in soil is therefore influenced by their availability for uptake by HCB.

Another novel result is that these bacterial species did not grow or exhibit any hydrocarbonoclastic activity in nitrogen-free media unless nitrogen gas was available.


Potential Hydrocarbonoclastic Bacteria

Advances in Applied Bioremediation. However, many molds and yeasts eukaryotes that are incapable of nitrogen fixation can also utilize hydrocarbons.

Pseudomonas stutzeri partial nifH gene for nitrogenase iron protein, strain Gr Some bacteria contain catabolic plasmids that can transform hydrocarbon pollutant into simple organic bbacteria plasmid genes encode for enzymes for ring cleavage and oxidation reactions. Join as an Editor-in-Chief. All five bacterial isolates manifested no lag phase of growth. In freshwater ecosystems, hydrocarbons form a thin hydrophobic layer after pollution.

Sequencing was performed in a xl genetic analyzer Applied Biosystems with sequencing analysis software version 5. This result corroborates with the report of Kaplan and Kitts The results obtained is relatively lower than that of Abdullahi et al.

None of the bacterial isolates exhibited a lag phase in their growth performances. Lipopolysaccharide produced in the bacterial membranes of gram negative bacteria supports the formation and stabilization of oil in aqueous system and contributes by increasing the attack surface on the pollutant hydrocarbonoclatsic subsequent assimilation Van Hamme et al. Retrieved from ” https: Hydrocarbonoclastic bacteria isolated from petroleum contaminated sites in Tunisia: Author Spotlight Raghuveer Singh.

Petroleum microbiology

These 15 bacterial species tested hydroczrbonoclastic for nitrogenase, as revealed by the GLC-detection of ethylene peaks in their culture top space gases GLC profiles not shown. Emulsification at this stage increases oil-water interface and faster rate of utilization and decomposition of oil substrates. Dion P, Nautiyal CS, editors.

Petroleum, crude oil or oil and hydrocarbons are sometimes used synonymously.